Tiller mortality and its relationship to grain yield in spring wheat

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Statistical Procedures
For the marked plant samples the data taken from TRS in 2010–2011 and 2011–2012 at BC in 2010–2011 and PRS in 2011–2012 were analyzed using a repeated measures design with the Proc Mixed procedure in SAS (SAS Institute, Inc., Cary, NC) to determine if there were differences in the number of spikes plant–1 and grain weight spike–1 among site-year, tiller initiation periods (planting to 31 December, 1 January to 28 February, and after 1 March) seeding rate, and N application timing. In all cases, site-year, seeding rate and N timing were treated as fixed effects, while blocks and the interactions with blocks were treated as random. When differences were detected, Fisher’s Protected LSD was used to separate means.

In the destructive sample plots some samples were lost in 2011–2012. Therefore, only samples taken in 2009–2010 and 2010–2011 at TRS and BC were used in the analysis. The Proc Mixed procedure in SAS (SAS Institute, Inc.) was used to determine if there were differences in spikes m–2, kernels spike–1, weight per 100 kernels, and grain yield among site-years,
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initiation period, seeding rate and N application timing. As with previous analysis, site-year, seeding rate, and N timing were treated as fixed effects; while blocks and the interactions with blocks were treated as random. When differences were detected, Fisher’s Protected LSD was used to separate means.

Grain yield from the large 18.01 m2 section of each plot for the 2010–2011 and 2011–2012 seasons at TRS and BC were analyzed using the Proc Mixed procedure in SAS (SAS Institute, Inc.) to determine if there were differences in grain yield among site-years, seeding rate, and N application timing. These site-years were chosen so that the grain yield from the large samples could be compared with that calculated from the small 2-m samples.
 
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